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. 1999 Oct 18;147(2):335–350. doi: 10.1083/jcb.147.2.335

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Electron microscopic analysis of kar3-64 kip3Δ cells. Wild-type (A) and kar3Δ kip3Δ (pkar3-64) cells (B–F) were arrested with hydroxyurea at 26°C, shifted to 35°C for 3 h, fixed, and sectioned for electron microscopy. A shows a normal preanaphase bipolar spindle. All the mutant spindles (B–F) appeared defective. In B and C, spindles have lost bipolarity. Although the poles are still slightly separated, they are not parallel. In D, E, and F spindles have collapsed with poles in a side by side orientation. All SPBs are marked with large arrowheads. Arrow in F marks a pole whose edge was just caught in this section. Small arrowheads mark cytoplasmic microtubules. n, nucleus. Bar, 0.2 μm. Strains used are listed in legend of Fig. 1.