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. 1999 Oct 18;147(2):335–350. doi: 10.1083/jcb.147.2.335

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Benomyl rescues the growth defects of cells expressing only CIN8 and either kar3-64 or kip3-14. Yeast cells were spotted onto solid rich media, with or without 10 μg/ml benomyl, and incubated at the indicated temperature for 3 d. The genotypes indicate the only two microtubule motor genes expressed in these cells (except for wild-type). Strains used (Note: p indicates a centromere plasmid-carried gene): wild-type (MAY1089), CIN8 pKAR3 (MAY5903), CIN8 pkar3-64 (MAY5904), CIN8 pKIP3 (MAY5905), CIN8 pkip3-14 (MAY5906), pCIN8 KAR3 (MAY5940), and pcin8-3 KAR3 (MAY5941).