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. 1999 Oct 18;147(2):321–334. doi: 10.1083/jcb.147.2.321

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9907129.f5a.jpg — Effects of dynactin subunit overexpression on γ tubulin distribution in Cos7 cells. (A) Representative images of cells double labeled with Abs to the transfected proteins (or imaged for GFP; left) and γ tubulin (right). The inset shows a cluster of γ tubulin foci enlarged 3×. Bar, 10 μm. (B) Pericentrin (left) and γ tubulin (right) staining in a cell overexpressing dynamitin (*). Arrowheads in A and B mark γ tubulin or pericentrin foci. (C) Cells overexpressing the protein of interest were scored as normal if they had one or two perinuclear γ tubulin foci. None, cells in the transiently transfected population that were not overexpressing protein; DM, dynamitin. Error bars indicate SD.

graphic file with name JCB9907129.f5b.jpg — Effects of dynactin subunit overexpression on γ tubulin distribution in Cos7 cells. (A) Representative images of cells double labeled with Abs to the transfected proteins (or imaged for GFP; left) and γ tubulin (right). The inset shows a cluster of γ tubulin foci enlarged 3×. Bar, 10 μm. (B) Pericentrin (left) and γ tubulin (right) staining in a cell overexpressing dynamitin (*). Arrowheads in A and B mark γ tubulin or pericentrin foci. (C) Cells overexpressing the protein of interest were scored as normal if they had one or two perinuclear γ tubulin foci. None, cells in the transiently transfected population that were not overexpressing protein; DM, dynamitin. Error bars indicate SD.