Table 1.

Summary of Effects of Dynactin Subunit Overexpression on Subcellular Organization

Class	Overexpressed protein	Microtubule array	Centrosomal p150	γ Tubulin	Centrosomal Arp1	Golgi	Dynactin structure	Pericentrin
—	Control (β-Gal)	95	93	89	84	85	Normal	93
A	Dynamitin	33	19	54	40	5	Disrupted	93
B	p150	20	ND	48	14	15	Normal	94
	CC1	28	22	46	30	7	Normal	95
C	CC2	33	37	49	89	88	Normal	95
	p24-GFP	20	39	47	85	80	Normal	94
—	p62	83	87	85	81	63	Normal	95

Cells were scored as described in Fig. 2 (microtubule array), 3 (dynactin structure), 4 (Golgi), 5 (γ tubulin) and 6 (centrosomal Arp1 and p150), or for a pericentriolar focus of pericentrin (right-most column). The percentage of cells showing a normal phenotype is given for each overexpression condition; standard deviations are provided in the figures. Dynactin structure was analyzed on sucrose gradients (Fig. 3) and was scored as normal if endogenous p150^Glued, p62, Arp1, and p24 cosedimented in a single peak at 20S. Dynactin shoulder/sidearm subunits are grouped into phenotypic classes (A, B, and C) as described in Discussion.