|
Fig. 2
|
Cells arrest in G2 of cycle 14 |
Two centriole pairs per cell: >95% |
| Cdc25string mutants |
while development continues well |
of pairs have incompletely |
|
after the time of mitosis 14 |
assembled daughters (n = 22) |
|
Fig. 3
|
<5% of cells in VE in metaphase (late stage 11) |
Centriole pairs disengage after metaphase |
| Wild type (late stage 11) |
|
|
| Cdc20fizzy mutants (late stage 11) |
>80% of cells in VE held up in metaphase |
62% of centriole pairs at the spindle poles are engaged (n = 21) |
| Cdc20fizzy mutants (early stage 12) |
>95% of cells in VE in mitosis |
100% centriole pairs disengaged |
| Cdc20fizzy mutants (midstage 12) |
>95% of cells in VE in mitosis |
100% centriole pairs disengaged |
|
Fig. 4
|
Most epidermal cells arrested in mitosis 14 |
100% centriole pairs disengaged (n = 17) |
| Heat shock promoter driven ectopic |
beyond their normal time of mitotic exit |
and 100% centrioles not associated with |
| expression of stable cyclins, A and B |
|
procentrioles (n = 39) |
| Control wild-type embryos exposed |
Development and cell cycle progression continues |
Procentriole formation not perturbed |
| to heat shock |
|
|
