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. 1999 Dec 27;147(7):1561–1568. doi: 10.1083/jcb.147.7.1561

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9907063.f1a.jpg — Decreased actin organization and increased migration of Shc expressing Cos-7 cells. (Left) Serum-deprived control and Shc stably expressing cells were harvested, plated on collagen-coated coverslips for 2 h, fixed, permeabilized, and stained with rhodamine phalloidin. The images shown are single confocal optical sections taken with a 63× objective (left). The scale bar is equal to 50 μm. (Right) Migration of parental vs Shc-expressing Cos-7 cells. Serum-deprived cells were allowed to migrate for 4 h in Boyden chambers towards a collagen matrix. Data from one of four independent experiments are shown (mean ± SE). The enhanced migration of the Shc cell line was found to be statistically significant (P < 0.05 by Student's t test). Random migration towards BSA-coated chambers was always <2% and subtracted from each value.

graphic file with name JCB9907063.f1b.jpg — Decreased actin organization and increased migration of Shc expressing Cos-7 cells. (Left) Serum-deprived control and Shc stably expressing cells were harvested, plated on collagen-coated coverslips for 2 h, fixed, permeabilized, and stained with rhodamine phalloidin. The images shown are single confocal optical sections taken with a 63× objective (left). The scale bar is equal to 50 μm. (Right) Migration of parental vs Shc-expressing Cos-7 cells. Serum-deprived cells were allowed to migrate for 4 h in Boyden chambers towards a collagen matrix. Data from one of four independent experiments are shown (mean ± SE). The enhanced migration of the Shc cell line was found to be statistically significant (P < 0.05 by Student's t test). Random migration towards BSA-coated chambers was always <2% and subtracted from each value.