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. 1999 Dec 27;147(7):1431–1442. doi: 10.1083/jcb.147.7.1431

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of HA-TIA-1ΔRRM on arsenite-induced assembly of SGs. COS cells were transiently transfected with recombinant HA-TIA-1ΔRRM, allowed to express protein for 48 h, and either untreated (A and C, paired views of same fields) or treated (B and D–F, paired views of same fields) with 0.5 mM sodium arsenite for 1 h before fixation and double staining for poly(A)⁺ RNA by in situ hybridization (C and D), and either HA-TIA-1ΔRRM (A, B, E) or endogenous TIAR (F) by immunofluorescence microscopy using mAbs reactive with the HA tag (A, B, and E) or endogenous TIAR (6E3). Arrows point out transfected cells. Bar, 10 μm.