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. 1999 Dec 27;147(7):1519–1532. doi: 10.1083/jcb.147.7.1519

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9904014.f5a.jpg — Specific role of LIMK1 and LIMK2 in focal complex formation induced by Rho family GTPases. A, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and Myc-tagged active form of Cdc42 (Cdc42V12; 50 μg/ml) were microinjected into serum-starved HeLa cells. B, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and the Myc-tagged active form of Rho (RhoV14; 50 μg/ml) were microinjected into serum-starved HeLa cells. C, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and Myc-tagged active form of Rac (RacV12; 50 μg/ml) were microinjected into serum-starved HeLa cells. 4 h later, these cells were fixed and double stained with anti-HA antibody (c and e), anti-Myc antibody (a), and antivinculin antibody (b, d, and f). Confocal images were obtained as described in Materials and Methods. The results shown are representative of three independent experiments. Bars, 20 μm.

graphic file with name JCB9904014.f5b.jpg — Specific role of LIMK1 and LIMK2 in focal complex formation induced by Rho family GTPases. A, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and Myc-tagged active form of Cdc42 (Cdc42V12; 50 μg/ml) were microinjected into serum-starved HeLa cells. B, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and the Myc-tagged active form of Rho (RhoV14; 50 μg/ml) were microinjected into serum-starved HeLa cells. C, Expression vectors for HA-tagged LIMK1/KD or LIMK2/KD (50 μg/ml) and Myc-tagged active form of Rac (RacV12; 50 μg/ml) were microinjected into serum-starved HeLa cells. 4 h later, these cells were fixed and double stained with anti-HA antibody (c and e), anti-Myc antibody (a), and antivinculin antibody (b, d, and f). Confocal images were obtained as described in Materials and Methods. The results shown are representative of three independent experiments. Bars, 20 μm.