Table 2.
MIBP Binds to the Membrane-proximal Region of the β1 Integrin Cytoplasmic Domain
| β1D integrin cytoplasmic domain deletion mutants | β1D integrin sequence | MIBP binding |
|---|---|---|
| Int β1D (749–801) | LIWKLLMIIHDRREFAKFEKEKMNAKW | + |
| DTQENPIYKSPINNFKNPNYGRKAGL | ||
| ΔBDMT1 (749–795) | LIWKLLMIIHDRREFAKFEKEKMNAKW | + |
| DTQENPIYKSPINNFKNPNY | ||
| ΔBDMT2 (749–785) | LIWKLLMIIHDRREFAKFEKEKMNAKW | + |
| DTQENPIYKS | ||
| ΔBDMT3 (749–777) | LIWKLLMIIHDRREFAKFEKEKMNAKW | + |
| DT | ||
| ΔBDMT4 (773–801) | ------------------------AKW | − |
| DTQENPIYKSPINNFKNPNYGRKAGL |
Interactions between proteins encoded by the pLexA and pB42AD constructs were determined by the activation of the reporter genes LEU2 and LacZ.