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. 1999 Dec 27;147(7):1409–1418. doi: 10.1083/jcb.147.7.1409

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Gel electrophoresis from different steps of nucleoskeleton preparations from stimulated human lymphocytes. (a) DNA was purified from agarose-embedded cells without cutting with enzymes (controls, 1), after cutting with EcoRI and HaeIII (2), and after cutting and removal of chromatin (3). Note the presence of nucleosomal bands in lane 2. Samples of DNA (1 μg in 1 and 2 and 0.5 μg in 3) were subjected to electrophoresis on a 1.7% agarose gel (M1: λ/EcoRI, HindIII; M2: pUCBM21/HpaII, DraI, HindIII). (b) Extracted fraction of chromatin after gel electrophoresis of cells cut with EcoRI and HaeIII. The two lanes displayed were loaded with roughly equal numbers of beads. Electrophoresis was performed on a 0.8% agarose gel that was subsequently treated with RNAse A and proteinase K (see Materials and Methods) before staining with ethidium bromide. (c) DNA hybridization of the blotted extracted fraction of chromatin using a human total genomic probe.