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. 1999 Dec 27;147(7):1409–1418. doi: 10.1083/jcb.147.7.1409

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Unstimulated human lymphocytes, control cells, and nucleoskeleton preparations hybridized with the centromeric alpha-satellite probe and the telomeric repeat probe. Nucleoskeleton preparations were produced by permeabilizing the agarose-encapsulated cells with 0.2% Triton X-100 and cutting of DNA with the restriction enzymes EcoRI and HaeIII. Afterwards, the cells were placed into the slots of an agarose gel, electroeluted, and used for in situ hybridization. All figures are projections of stacks of optical sections. (a) Control cell, centromeric probe; (b) nucleoskeleton preparation, centromeric probe; (c), control cell, telomeric probe; (d) nucleoskeleton preparation, telomeric probe. Bar, 2 μm.