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. 1999 Dec 27;147(7):1409–1418. doi: 10.1083/jcb.147.7.1409

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Stimulated human lymphocytes, control cells, and nucleoskeleton preparations hybridized simultaneously with centromeric alpha-satellite probe and ribosomal DNA probe, or with telomeric repeat probe and ribosomal DNA probe. (a) Control cell, centromeric probe detected with FITC-labeled avidin and A fragment detected with rhodamine-labeled antibodies; (b) nucleoskeleton preparation, hybridization protocol as in a; (c) control cell, D_HH fragment detected with FITC-labeled avidin, telomeric probe detected with rhodamine-labeled antibodies; (d) nucleoskeleton preparation, hybridization protocol as in c. Bar, 2 μm.