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. 1999 Dec 27;147(7):1493–1502. doi: 10.1083/jcb.147.7.1493

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9906064.f7a.jpg — Effect of Bax and ANT-1 expression on the growth of yeast cells. (A) Bax but not ANT-1 leads to growth inhibition in yeast. Bax and ANT-1 cloned in expression vectors under the control of a galactose and raffinose-inducible promoter and the empty control vector were introduced into yeast cells. 4.5 × 10⁴ yeast cells of three independent clones each were plated on glucose (glu)- or galactose and raffinose (gal/raf)–containing agar. The growth of the yeast cells was monitored 36 h later. (B) Control of inducible expression of ANT-1 and Bax in yeast cells. Yeast clones from A were kept in normal medium or incubated in galactose and raffinose–containing medium for 8 h. Extracts were prepared, and equal amounts (40 μg) of proteins were investigated for the expression of Bax and ANT-1 in a Western blot. The antiserum against ANT-1 does also recognize the endogenous yeast ANT-1.

graphic file with name JCB9906064.f7b.jpg — Effect of Bax and ANT-1 expression on the growth of yeast cells. (A) Bax but not ANT-1 leads to growth inhibition in yeast. Bax and ANT-1 cloned in expression vectors under the control of a galactose and raffinose-inducible promoter and the empty control vector were introduced into yeast cells. 4.5 × 10⁴ yeast cells of three independent clones each were plated on glucose (glu)- or galactose and raffinose (gal/raf)–containing agar. The growth of the yeast cells was monitored 36 h later. (B) Control of inducible expression of ANT-1 and Bax in yeast cells. Yeast clones from A were kept in normal medium or incubated in galactose and raffinose–containing medium for 8 h. Extracts were prepared, and equal amounts (40 μg) of proteins were investigated for the expression of Bax and ANT-1 in a Western blot. The antiserum against ANT-1 does also recognize the endogenous yeast ANT-1.