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. 1999 Dec 27;147(7):1443–1456. doi: 10.1083/jcb.147.7.1443

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9805090.f7a.jpg — PDI and BiP are associated in the ER lumen and dissociate in the presence of a folding substrate. a, Wild-type and pdi1 mutant microsomes were incubated in translocation reactions containing pΔgpαf (lanes 1–3) or no translocation substrate (lanes 4–6). At the end of the translocation, DSP cross-linking was performed as described above, followed by solubilization of the membranes, and immunoprecipitation with anti-PDI antiserum. Cross-links in the immunoprecipitate were cleaved with DTT, proteins were resolved on 7.5% SDS gels, and transferred to nitrocellulose membranes. BiP was detected by immunoblotting with anti-BiP antiserum (1:1,000) followed by incubation with ¹²⁵I-protein A and PhosphorImaging. b, Quantitation of the experiment shown in a.

graphic file with name JCB9805090.f7b.jpg — PDI and BiP are associated in the ER lumen and dissociate in the presence of a folding substrate. a, Wild-type and pdi1 mutant microsomes were incubated in translocation reactions containing pΔgpαf (lanes 1–3) or no translocation substrate (lanes 4–6). At the end of the translocation, DSP cross-linking was performed as described above, followed by solubilization of the membranes, and immunoprecipitation with anti-PDI antiserum. Cross-links in the immunoprecipitate were cleaved with DTT, proteins were resolved on 7.5% SDS gels, and transferred to nitrocellulose membranes. BiP was detected by immunoblotting with anti-BiP antiserum (1:1,000) followed by incubation with ¹²⁵I-protein A and PhosphorImaging. b, Quantitation of the experiment shown in a.