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. 1999 Dec 27;147(7):1365–1370. doi: 10.1083/jcb.147.7.1365

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TPA induces MMP-13 and MMP-3 in skin. a, TPA (10 nmol) in acetone was applied to the back skin. After 0, 1, 4, and 6 h, mice were killed and RNA from skin was prepared. MMP-13, MMP-3, c-jun, and c-fos expression was analyzed by Northern blot analysis. b, TPA (0.1, 1, 10, and 50 nmol) was applied as described in a, and animals were killed after 6 h and analyzed by Northern blot analysis. Rehybridization with a cDNA fragment of 18 S RNA was performed serving as a loading control.