Figure 1.

Bar chart of relative NF-κB activation (luciferase activity divided by β-galactosidase activity) in E18 trigeminal neurons under different experimental conditions. The neurons were initially cultured with NGF for 24 h, washed, and incubated in NGF-free medium for 2 h, then coinjected with the NF-κB–dependent luciferase reporter construct and the RSV lacZ expression plasmid (plus the super-repressor IκB-α expression plasmid in the cultures indicated), and maintained for a further 6 h before assaying for luciferase and β-galactosidase activities. CNTF (50 ng/ml) was added to the cultures indicated immediately after injection. Relative NF-κB activity at each point is calculated from the ratio of luciferase to lacZ activity compared with the untreated control. The mean and SEM are shown for three separate experiments.