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. 2000 Jan 24;148(2):317–324. doi: 10.1083/jcb.148.2.317

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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AFM imaging of membrane patches, imaged in air using tapping mode. A and B show two membrane patches representative of those used in the in vitro assay for exocytosis. Boxed areas in A and B are magnified in C and D, respectively. C and D show individual particles. Dotted lines denote the line of cross-section shown above C and D. The mean particle height was 34 ± 0.4 nm and the mean diameter was 225 ± 2 nm (means ± SEM, n = 77). AFM images are black/white-coded with respect to height; a height scale is shown on the right. Bars: (A and B) 2 μm; (C and D) 500 nm.