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. 2000 Mar 20;148(6):1213–1222. doi: 10.1083/jcb.148.6.1213

Figure 2.

Figure 2

Sequence analysis of Toc64. (a) Protein sequence of pea Toc64 as deduced from the cDNA clone. The determined amino acid sequences are underlined. These sequence data are available from GenBank/EMBL/DDBJ under accession no. AF179282. (b) Sequence comparison of pea (Pis) Toc64 with a Toc64 homologue from Arabidopsis thaliana (Ara; sequence data available from EMBL/GenBank/DDBJ under accession no. H36863), amidases (amid) from Arabidopsis thaliana (sequence data available from EMBL/GenBank/DDBJ under accession no. AC000106), Rhodococcus rhodochrous (Rho; sequence data available from EMBL/GenBank/DDBJ under accession no. D16207), and Synechocystis sp. (Syn; sequence data available from EMBL/GenBank/DDBJ under accession no. D90907), Aquifex aeolicus glutamyl-tRNAGln amidotransferase subunit A (Aqu Glu-AdT; sequence data available from EMBL/GenBank/DDBJ under accession no. AE000680), and Pseudomonas syringae indoleacetamide hydrolase (Pse IAH; sequence data available from EMBL/GenBank/DDBJ under accession no. U04358) using the ClustalW 1.7 and BOXSHADE programs. The active residues of the amidases are indicated by asterisks. The arrow shows the amino acid exchange of Toc64. (c) Sequence alignment of TPR motifs of different polypeptides: pea Toc64, its homologue from Arabidopsis thaliana, hop from Rattus norvegicus (Rat; sequence data available from EMBL/GenBank/DDBJ under accession no. CAA75351) and its plant homologue Sti1 from Glycine max (Gly; sequence data available from EMBL/GenBank/DDBJ under accession no. S56658), human hTom34p (Hum; sequence data available from EMBL/GenBank/DDBJ under accession no. U58970), Podospora anserina (Pod) Tom70 (sequence data available from EMBL/GenBank/DDBJ under accession no. Y14750), and serine/threonine protein phosphatase 5 (PP5) from Rattus norvegicus (sequence data available from EMBL/GenBank/DDBJ under accession no. P53042) using the ClustalW 1.7 and BOXSHADE programs. (d) Hydropathy analysis of Toc64 according to Kyte and Doolittle 1982 using a window size of 19 amino acids.