Table 1.
Comparison of the Relative Induction and Activation of Matrilysin by Bacteria, Cytokines, and Other Stimuli
| Condition | Concentration | Fold induction | Matrilysin activation |
|---|---|---|---|
| ng/ml | |||
| No stimulus | − | ||
| Adherent bacteria | 50–75 | + | |
| Nonadherent bacteria | 5–10 | ± | |
| IL-1β | 10–25 | 12 | − |
| IL-6 | 25–50 | 8 | − |
| TNFα | 25–50 | 7 | − |
| EGF | 100 | 3 | − |
| Phorbol ester | 10–50 | 3 | − |
| LPS | 1,000 | ne | − |
| IFN-γ | 50–100 | ne | − |
| IL-4 | 20–50 | ne | − |
| HSF/SF | 30–60 | ne | − |
| GM-CSF | 50 | ne | − |
| IL-8 | 100 | ne | − |
| MCP-1 | 50–100 | ne | − |
| TGF-β1 | 100 | ne | − |
HT29 human colon epithelial cells were plated on 6-well plates and infected with bacteria (300 bacterial/cell) or treated with the indicated concentrations of cytokines and other stimuli. Western blotting was performed on conditioned media collected 48 h after infection or 48 h after addition of the other stimuli. Autoradiograms were analyzed by densitometry, and the results are expressed relative to the values of control cells (ne, no effect). (+) 50% or more activated matrilysin; (±) <50% activated matrilysin; and (−) no activated matrilysin.