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. 2000 Mar 20;148(6):1283–1294. doi: 10.1083/jcb.148.6.1283

Figure 3.

Figure 3

Cell adhesion activity of the recombinant neuropilin-1 proteins. (A) A schematic representation of the Fc-tagged full-length neuropilin-1 extracellular segment (NP-Fc) and the b1-b2 domains–deleted one [NP(b-)-Fc], and GST-tagged recombinant b1 (GST-b1) and b2 (GST-b2) domains. (B) SDS-PAGE of the recombinant proteins stained with Coomassie brilliant blue G-250. Lanes 1–5 correspond to NP-Fc, NP(b-)-Fc, GST, GST-b1, and GST-b2 recombinant proteins, respectively. (C) Cell adhesion to the recombinant proteins immobilized on nitrocellulose-coated culture dishes. L cells adhere to Fc-NP, GST-b1, and GST-b2 but not NP(b-)-Fc and GST. Bar, 100 μm.