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Binding of AP-tagged semaphorin 3A (SEMA3A-AP) to mutant neuropilin-1 proteins. Binding of SEMA3A-AP to the transfectants expressing NP-full 86 (A; see Fig. 1 B), NP-bc 30 (B; see Fig. 1 B), NP-ac 24 (C; see Fig. 2 B), NP-abm 716 (D; see Fig. 2 C), NP-am 38 (E; see Fig. 2 C), and NP-c 68 (F; see Fig. 1 B). SEMA3A-AP bound to cell surface was visualized with NBT/BCIP. Bar, 100 μm.
