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. 2000 Mar 20;148(6):1165–1176. doi: 10.1083/jcb.148.6.1165

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of ubinuclein transcript in human tumor tissues. A, Tumor tissue cDNA panel was used as a PCR template as described in Fig. 3. Lane 1, Molecular weight marker, 100-bp ladder; lane 2, negative control; lane 3, colon adenocarcinoma (GI-112); lane 4, colon adenocarcinoma (CX-1); lane 5, pancreatic adenocarcinoma (GI-103); lane 6, prostatic adenocarcinoma (PC-3); lane 7, lung carcinoma (GI-117); lane 8, lung carcinoma (LX-1); lane 9, breast carcinoma (GI-101); lane 10, ovarian carcinoma (GI-102). B, Northern blot analysis of human mRNA. Hybridization of the human cancer cell line multiple tissue Northern blot with the ubinuclein-specific probe revealed a transcript of 7 kb. G3PDH-specific probe was used as a control. Lane 1, Promyelocytic leukemia HL-60; lane 2, HeLa cells S3; lane 3, chronic myelogenous leukemia k-562; lane 4, lymphoblastic leukemia MOLT-4; lane 5, colorectal adenocarcinoma SW40; lane 6, lung carcinoma A549; lane 7, melanoma G361.