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. 2000 Mar 20;148(6):1165–1176. doi: 10.1083/jcb.148.6.1165

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Western blot analysis of the endogenous ubinuclein and the polypeptides expressed from the transiently transfected expression constructs. Total extracts of HaCaT keratinocytes (control) and of those transfected with the Ubi-F, Ubi-N, and Ubi-Z/ZAP5 expression constructs were separated on a 10% SDS-PAGE and detected with ZAP5 antibody (top). The same blot was stained with antiactin antibody (bottom). The arrows point to the bands corresponding to the full-length ubinuclein (top), and polypeptides encoded by Ubi-Z/ZAP5 (middle) and Ubi-N (bottom) expression constructs.