Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Mar 20;148(6):1231–1238. doi: 10.1083/jcb.148.6.1231

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Vam2/6p is associated with the cis-SNARE complex. (A) Association of Vam2p with Vam3p. BJ3505 vacuoles were incubated in 10× scale fusion reactions at 27°C for 10 min with or without ATP. Lane 3 was supplemented with antibody to Sec18p. Vacuoles were solubilized in buffer containing 1% Triton X-100 as described in Materials and Methods, and proteins immunoprecipitated from the detergent extracts with antibody to Vam3p. Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting with antisera to the indicated vacuolar proteins. (B) Vam2p is associated with Vti1p and Ytk6p. An immunoblot of vacuole protein immunoprecipitates from Ungermann et al. 1999 was probed with antibody to Vam2p.