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Vacuole priming dissociates the Vam2/6p complex. BJ3505 wild-type vacuoles (300 μg) were incubated at 27°C for 10 min with or without Mg2+/ATP in standard fusion reactions. Vacuoles were then sedimented, solubilized in PS buffer containing 1% Triton X-100, and analyzed on sucrose velocity gradients as in Fig. 1.
