Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Mar 20;148(6):1223–1230. doi: 10.1083/jcb.148.6.1223

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Vam2p and Vam6p are associated with purified vacuoles. A BJ3505 wild-type culture was processed for vacuole purification (Haas 1995). An aliquot of spheroplasted yeast cells was lysed by boiling in SDS-PAGE loading buffer, and the remaining spheroplasts were carried through the vacuole purification. Equivalent proportions of spheroplasts and purified vacuoles (0.1% of the total preparation) were analyzed by SDS-PAGE and immunoblotting with antisera against the indicated proteins. Recovery of proteins in the purified vacuoles was determined by scanning densitometry (see text).