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. 2000 Mar 20;148(6):1223–1230. doi: 10.1083/jcb.148.6.1223

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Kinetics of acquisition of resistance to Gdi1p and antibodies to Vam3p, Vam2p, and Vam6p. Aliquots (30 μl) were removed from 10× scale fusion reactions at the indicated times and added to control buffer on ice, buffer at 27°C, Gdi1p (64 μg/ml), or antibodies against Sec18p, Vam3p, Vam2p, or Vam6p at 27°C. Fusion reactions were incubated for a total of 90 min at 27°C before being assayed for alkaline phosphatase activity.