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. 2001 May 28;153(5):1071–1084. doi: 10.1083/jcb.153.5.1071

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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MLCK inhibitor ML-7–induced growth cone collapse in CM correlates with actin bundle loss in PA growth cones. When ML-7 was added onto growth cones cultured in CM, it induced significant growth cone collapse (B) and neurite retraction (arrows) compared with before application (A). When PA growth cones (D) were treated with ML-7, actin bundle loss occurred (E). Both effects were reversible. Growth cones in CM and actin bundles in PA growth cones reformed after washing out the ML-7 (C and E). ML-7–induced actin bundle loss was dose and time dependent (G and H). *P < 0.0005, significant difference from control; #P < 0.005, significant difference from 5 μM ML-7. Bar: (A–C) 40 μm; (D–F) 20 μm.

MLCK inhibitor ML-7–induced growth cone collapse in CM correlates with actin bundle loss in PA growth cones. When ML-7 was added onto growth cones cultured in CM, it induced significant growth cone collapse (B) and neurite retraction (arrows) compared with before application (A). When PA growth cones (D) were treated with ML-7, actin bundle loss occurred (E). Both effects were reversible. Growth cones in CM and actin bundles in PA growth cones reformed after washing out the ML-7 (C and E). ML-7–induced actin bundle loss was dose and time dependent (G and H). *P < 0.0005, significant difference from control; #P < 0.005, significant difference from 5 μM ML-7. Bar: (A–C) 40 μm; (D–F) 20 μm.