Table 2.
Phenotypic Characterization of Multicolony-derived Strains of Human Marrow Stromal Cells and Guinea Pig Blood-derived Adherent Cells, and of Single Colony–derived Strains of Human and Rabbit Blood-derived Adherent Cells
| Marker | Rabbit polygonal non-osteogenic | Rabbit fibroblast-like osteogenic | Guinea pig osteogenic | Human fibroblast-like non-osteogenic | Human fibroblast-like osteogenic | Human marrow stromal cells | |
|---|---|---|---|---|---|---|---|
| Fibroblastic | |||||||
| Col I (LF41) | ++ | + | ++ | ++ | ++ | ++ | |
| Col III (LF71) | −/+ | −/+ | + | + | + | ++ | |
| Fibronectin (rabbit; ICN Biomedicals) | ++ | ++ | |||||
| Fibronectin (guinea pig, MsGP13; Serotec) | ++ | ||||||
| CD44 (H9H11) | ++ | ++ | ++ | ||||
| β1 integrin subunit (MAB1959, Chemicon) | ++ | +/++ | ++ | ||||
| Osteogenic | |||||||
| ON (BON-1) | ++ | +/++ | ++ | −/+ | ++ | ++ | |
| OPN (LF123) | +/++ | + | ++ | −/+ | −/+ | −/+ | |
| BSP (LF83) | +/++ | + | + | − | − | −/+ | |
| OC (LF32) | −/+ | −/+ | −/+ | ||||
| Adipocytic | |||||||
| CEBPα (Santa Cruz Biotechnology, Inc.) | −/+ | −/+ | −/+ | ||||
| PPARγ2 (Santa Cruz Biotechnology, Inc.) | − | − | − | − | − | ||
| Endothelial | |||||||
| CD34 (Dako) | − | − | − | ||||
| Muc-18 | − | − | + | ||||
| Factor VIII (Dako) | − | − | − | ||||
| Endoglin | − | − | + | ||||
| PAL-E (rabbit; Accurat Chemical Scientific) | − | − | |||||
| EN4 (guinea pig; Accurat Chemical Scientific) | − | ||||||
| Marrow stromal cells | |||||||
| Stro-1 | − | − | −/+ | ||||
| CD106 (VCAM-1) (human, MCA907; Serotec) | ++ | ++ | + | ||||
| Smooth muscle | |||||||
| α-Smooth muscle actin (1A4; Dako) | + | ++ | ++ | ++ | ++ | −/+/++ | |
| Smooth muscle (rabbit and guinea pig, ACL-10002; Accurat Chemical Scientific) | −/+ | + | +/++ | ||||
| Skeletal muscle | |||||||
| MyoD (M318; Dako) | − | − | − | ||||
| Monocyte/macrophage | |||||||
| CD14 (human, TUK4; Dako) | − | − | − | ||||
| RAM11 (rabbit; Dako) | − | − | |||||
| MR-1 (guinea pig; Serotec) | − | ||||||
| Leukocyte | |||||||
| CD45 (human, 2B11/PD7-26; Dako) | − | − | − | ||||
| CD45 (rabbit, L12/201; Serotec) | − | − | |||||
| CD45 (guinea pig, 1H-1; Serotec) | − | ||||||
| Nerve | |||||||
| Neurofilament (2F11; Dako) | − | − | − | ||||
| Histochemical | |||||||
| Alkaline phosphatase | − | − | − | − | − | −/+/++ | |
| Acid phosphatase | + | + | + | + | + | + | |
| α-Naphthyl acetate esterase | − | − | +/++ | +/++ | + | ||
| Oil Red O (lipid accumulation) | |||||||
| 20% FBS | −/+ | − | −/+ | −/+ | + | −/+ | |
| 20% Rabbit serum | +/++ | −/+/++ | ++ | ++ | ++ | ++ | |
++, intensive staining; +, weak staining; −, negative staining.