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. 2001 May 28;153(5):1127–1132. doi: 10.1083/jcb.153.5.1127

Figure 1.

Figure 1

MuSK cross-link products and immunopurification of MuSK complexes. (a) Proteins from Torpedo AChR-rich membranes were cross-linked with various concentrations of SMPB (lane 0, control; lane 1, 7 × 10−6 M; lane 2, 2 × 10−5 M; lane 3, 4 × 10−5 M; lane 4, 10−4 M; lane 5, 2.5 × 10−4 M) and separated by SDS-PAGE. Western blots revealed that in addition to MuSK (97 kD), a 140-kD cross-link product was detected at low SMPB concentrations. Other cross-link products were observed at higher concentrations. (b) Purification of uncross-linked (−SMPB) and cross-linked (+SMPB, 4 × 10−5 M) MuSK complexes was achieved by immunoaffinity chromatography. The immunopurified polypeptides or the MuSK cross-link products were detected by silver staining (ST) or identified by immunoblotting (IB), respectively. In both experiments, a 40-kD polypeptide was detected in addition to MuSK. After SMPB treatment, a major cross-link product of 140 kD was detected. The asterisk indicates residual IgGs.