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. 2001 May 28;153(5):1035–1048. doi: 10.1083/jcb.153.5.1035

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Control experiment: photoactivation of caged FITC-dextran in the wing of the keratocyte. (A) Positive rhodamine fluorescence identifies a loaded cell. (B) Before uncaging, the fluorescence in the FITC channel was negligible, as expected. (C) After photoactivation, caged FITC-dextran was uncaged successfully as demonstrated by FITC fluorescence throughout the cell. (D–G) Time-lapse microscopy of the same cell shows unaltered locomotion as a result of such photorelease (the 3-μm zone of photoactivation is indicated by a circle).