Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 May 28;153(5):1011–1022. doi: 10.1083/jcb.153.5.1011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

A1 is a GSE attenuating CHOP expression by ER stress and amino acid deprivation. (A) GFP expression levels analyzed by FACS^® in parental CHOP::GFP-containing CHO cells and in cells transduced by the A1 retroviral clone. Cells were untreated and cultured in complete media (UT), cultured for 16 h in media lacking leucine (−Leu), or exposed to tunicamycin (2 μg/ml) for 16 h (Tm). The fold increase in GFP compared with the untreated cells is indicated. (B) Immunoblot of endogenous CHOP in the parental and A1-transduced cells after exposure to tunicamycin (2 μg/ml) or leucine-free media (upper panel). Anti-eIF2α immunoblot of the same membrane serves as a control of protein loading (lower panel).