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. 2001 May 28;153(5):999–1010. doi: 10.1083/jcb.153.5.999

Figure 5.

Figure 5

Inhibition of cathepsin B activity or expression protects WEHI-S cells from death receptor–induced apoptosis. (A) WEHI-S cells were pretreated with indicated protease inhibitors for 1 h before the addition of 100 pg/ml rhTNF. The survival of the cells was analyzed 12 h later by the MTT assay. (B) WEHI-S cells were treated for 11 h with indicated concentrations of CA-074-Me and rhTNF in the presence or absence of 1 μM zVAD-fmk or 10 μM cycloheximide (CHX). The cytotoxicity was measured by the LDH release assay. (C) The survival of WEHI-S cells pretreated with CA-074-Me for 1 h before the treatment with cytotoxic agents, as indicated in the legend for Fig. 1 E (except for TRAIL which was used at 150 ng/ml) was measured by the MTT assay. (D) WEHI-S cells were transfected with indicated plasmids plus pEGFP-N1 and treated 22 h later with rmTNF alone or with 1 μM zVAD-fmk or 20 μM CA-074-Me. The survival of green cells was determined 12 h later. The values represent means of triplicate determinations (A–C) or means of 10 (D) randomly chosen fields of ≥100 cells ± SD. *p-value < 0.01, as compared with control cells (A and C) or to similarly treated vector-transfected cells (D).