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. 2000 Nov 27;151(5):1047–1056. doi: 10.1083/jcb.151.5.1047

Figure 2.

Figure 2

Distribution of Mcd1p-binding sites on RDN1 and neighboring unique and intermediate repetitive sequences on chromosome XII. (A) A physical map of the RDN1 repeat unit showing various sequence features. R1 and R45, PCR fragments; NTS1 and NTS2, nontranscribed spacers 1 and 2. (B) An Mcd1p-binding site on the repetitive rDNA locus, RDN1, on chromosome XII. PCR analysis of total or Mcd1p coimmunoprecipitated DNA (HA) from α-factor or nocodazole arrested cell lysates of a region of the rDNA repeat, including the Mcd1p-binding site (PCR fragments R39–R45). PCR fragments shown are R36, R37…R44, R45, and R1…R5. (C) Mcd1p binding profile on a region of chromosome XII including a single-copy region, RDN1, and an IR. The physical map of the selected region shows ORFs and other sequence features. Two copies of the RDN1 repeat are shown for clarity, assuming that a majority of repeats are occupied. The amount of DNA immunoprecipitated estimated as the percent of total DNA is plotted on the y axis versus the position of the midpoint of each fragment on chromosome XII in bp on the x axis.