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. 2000 Nov 27;151(5):1101–1112. doi: 10.1083/jcb.151.5.1101

Figure 1.

Figure 1

Phenotypic characterization of the mal25-1 strain. (A) Sectoring phenotypes of wild-type (wt) and mal25-1 strains grown on indicator plates (Fleig et al. 1996) for 6 d at 24°C. (B) Serial dilution patch test for sensitivity to the microtubule-destabilizing drug TBZ. Dilutions shown were 10-fold. Although the isogenic wild-type strain (b and d) only shows slightly reduced growth at 24°C on rich medium containing 7 μg/ml TBZ, the mal25-1 strain (a and c) is unable to grow. (C) The mal25-1 strain shows growth defects at higher temperatures. Serial dilution patch tests of wild-type strain (a, c, and e, grown at 24°C, 30°C, and 36°C, respectively) and mal25-1 strain (b, d, and f grown at 24°C, 30°C, and 36°C, respectively) are shown. (D) Photomicrographs of wild-type (insert) and mal25-1 strain show an abnormal, elongated cell form of mutant strain. At 30°C, wild-type cells had an average length of 13.2 ± 1.2 μm at septa formation, whereas mal25-1 cells were 19.0 ± 3 μm in length. Arrows indicate septum. Bar, 10 μm.