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. 2000 Nov 27;151(5):1101–1112. doi: 10.1083/jcb.151.5.1101

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Nucleocytoplasmic transport is normal in spi1-25 cells. Wild-type or spi1-25 cells with either integrated GFP-SV40, NLS-LacZ, or GFP-pap1 were grown to midlog phase at 25°C then shifted to 36°C for 4 h. The GFP-SV40 NLS-β-galactosidase reporter protein is exclusively nuclear localized in wild-type (A) and spi1-25 cells (B). Without hydrogen peroxide treatment, GFP-Pap1p is exported from the nucleus in both wild-type (C) and spi1-25 cells (D). 15 min after the addition of 0.8 mM hydrogen peroxide, the fusion protein is imported into the nucleus in wild-type cells (E) and spi1-25 cells (F). Bar, 10 μm.

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