Figure 5.
Antitubulin immunofluorescence images of interphase spi1-25 cells show aberrant cytoplasmic microtubule cytoskeleton. (a–d) spi1-25 cells cultured at 30°C; (e) a wild-type cell. Each cell is depicted as a phase–contrast image to visualize cell shape followed by two antitubulin immunofluorescence images representing two different focal planes. Although the cytoplasmic microtubules of wild-type cells (e) are aligned along the long axis of the cell reaching the cell tips, spi1-25 cells frequently have microtubules that fail to reach the cells tips (a and b) and in some cases are much shorter than wild-type interphase microtubules (c) or have a single microtubule bundle on one side of the cell (d) and cells bend away from this bundle. Bar, 10 μm.