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. 2000 Nov 27;151(5):1101–1112. doi: 10.1083/jcb.151.5.1101

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The mitotic spindle checkpoint is activated in spi1-25 cells. (A) Serial dilution patch tests (10⁴ to 10¹ cells) show growth of mph1Δ and spi1-25 single mutants and spi1-25 mph1Δ double mutant at 25°C on rich medium for 4 d. The strains all come from the same tetrad. (B) Diagrammatic representation of chromatin missegregation in the strains described in A. Cells were grown at 30°C for 12 h and fixed, and the chromatin was analyzed by staining with DAPI. Given are the percentages for the various abnormal chromatin segregation phenotypes in an asynchronously growing cell population. n.o., not observed.

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