Figure 8.
Phenotypic analysis of the spi1-25 mal3Δ double mutant. (A) Serial dilution patch tests (104 to 101 cells) of wild-type (spi1 + mal3 +), single mutant (mal3Δ or spi1-25), and double mutant (spi1-25 mal3Δ) strains grown on rich medium with or without TBZ at 25°C. (B) The percentage of cells with abnormal cell morphology as shown diagrammatically was determined for the indicated strains. Strains were grown asynchronously in rich liquid medium at 25°C. Indicated is an abnormal cell type not seen in the single mutant strains (right).