Table 2.
spi1-25 Interactions
| Two-hybrid interactions | |||
|---|---|---|---|
| Gene | Function | Interaction | |
| nda2+ | α-Tubulin | No | |
| sbp1+ | Ran-GTP BP | Yes | |
| Genetic interactions | |||
| Gene | Function | Phenotype | |
| pim1d | Ran GEF | Synthetic lethal | |
| cut11-2 | SPB component | Synthetic lethal | |
| cut11-3 | SPB component | Synthetic lethal | |
| cut11-7 | SPB component | Poor growth at 24°C, dead at 30°C | |
| mph1Δ | Spindle checkpoint component | Extremely poor growth at 24°C; increase in chromosome missegregation | |
| mad2Δ | Spindle checkpoint component | None | |
| mal3Δ | MAP | Very poor growth at 24°C; dead on sublethal doses of TBZ; increase in abnormal cell form; increase in chromosome missegregation | |
| Multicopy suppression | |||
| Overexpression | Function | Allele to be rescued | Phenotype |
| spb1+ | Ran-GTP BP | spi1-25 | Lethal as for spi1+ |
| rna1+ | Ran-GAP | spi1-25 | Lethal as for spi1+ |
| spi1+ | Ran | pim1d | Good rescue |
| spi1-25 | Ran | pim1d | Moderate rescue |