Table 2.

Single Molecule Motility of Kinesin Coiled-Coil Mutants on Axonemes

		Run length
Construct	n	Observed	Corrected	Velocity
		μm	μm	μm/s
Wild type	246	1.1 ± 0.1	1.5 ± 0.2	0.389 ± 0.005
H1Q	108	2.5 ± 0.3	6.6 ± 2.6	0.371 ± 0.006
H5Q	156	1.7 ± 0.2	2.5 ± 0.3	0.460 ± 0.007
5Lys	128	1.9 ± 0.2	3.5 ± 0.8	0.392 ± 0.008
4Glu	172	0.28 ± 0.03	0.30 ± 0.03	0.345 ± 0.008

Single molecule velocities and run lengths were measured as described in the Materials and Methods. n is the number of events that were scored for each mutant. Errors were determined by bootstrapping and are reported as 1 SD. All measurements were repeated on two separate preparations and agreed within error. The numbers reported here are derived from a single fit of all measured data. Observed run lengths were corrected for photobleaching as described in the Materials and Methods. For all mutants, the probability was <10⁻⁴ that the measured run lengths were distributed the same as the wild-type run lengths (determined using the Kolmogorov-Smirnov test).