Table 4.
Single Molecule Motility of Kinesin Mutants on Microtubules, Before and After Subtilisin Treatment
| Run length | |||||
|---|---|---|---|---|---|
| Construct | Substrate | n | Observed | Corrected | Velocity |
| μm | μm | μm/s | |||
| Wild type | MT | 54 | 0.8 ± 0.2 | 1.0 ± 0.2 | 0.52 ± 0.02 |
| Wild type | SMT | 73 | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.40 ± 0.06 |
| H1Q | MT | 60 | 2.9 ± 0.3 | 5.8 ± 1.6 | 0.51 ± 0.02 |
| H1Q | SMT | 43 | 0.8 ± 0.1 | 1.5 ± 0.5 | 0.25 ± 0.02 |
Single molecule measurements were made as described in Materials and Methods and Table . n is the number of events that were scored for each mutant. Run lengths were significantly different on subtilisin-treated microtubules compared with untreated microtubules (P < 2 × 10−3) for both wild type and H1Q.