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. 2000 Nov 27;151(5):973–984. doi: 10.1083/jcb.151.5.973

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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α-Factor and Pma1p packaging into vesicles reconstituted with cytosol. Budding assays were performed in duplicates as described in Materials and Methods using donor membranes from lst1Δ, HA-Pma1p-containing cells primed with wild-type cytosol, or lst1Δ cytosol supplemented with Sec23/Lst1p as indicated. Percent packaging of α-factor and Pma1p was determined as described in Materials and Methods and is reported as the mean of at least two independent experiments with error bars indicating SD.