Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Nov 27;151(5):973–984. doi: 10.1083/jcb.151.5.973

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Coimmunoprecipitation of Lst1p and Sec24p is membrane-dependent. Reactions containing purified COPII components and a mixture of both Sec23/Lst1p and Sec23/24p (lanes 1) or Sec23/24p only (lanes 2) were set in the presence (+memb) or absence (−memb) of donor membranes and supernatants containing vesicles (+memb) or only soluble COPII proteins (−memb) were cross-linked and centrifuged as described under Materials and Methods. Solublized pellets were then either immunoprecipitated with anti-Lst1p serum and immunoblotted with anti-Sec24p serum (A) or vice versa (B). Purified Sec23/24p and Sec23/Lst1p were also separated on the gel for immunoblotting as indicated.