Figure 1.

Verification of the HPLC procedure. (A) XAR activity of the HPLC fractions F1 (0.00–5.30 min; without endogenous hormones) and F2.1 (5.30–12.0 min; with endogenous hormones) from three different males were determined in the AR-CALUX assay. (B) Mean ± SE of the F1 fraction of KHM and KHF serum controls alone (XAR; n = 12) or in the presence of R1881 EC₅₀ (XARcomp; n = 9).

*XAR activity of the F2.1 fraction significantly higher than for the F1 fraction. ^#Significantly lower than the R1881 EC₅₀ solvent control.