Figure 2.

Dose–response ER transactivation of E₂ and the four test chemicals. (A) The MVLN cells were exposed to E₂ in the concentration range of 0.05–500 pM and to the test chemicals at 10⁻⁸–10⁻⁴ M for 24 hr. Solvent control was set to 100%. E₂ EC₁₀₀ and EC₅₀ as well as EC₅₀ for each of the test chemicals were determined by Sigma Plot 8.0. (B) Agonistic and antagonistic ER activity of test chemicals BPA, BPA-DM, nNP, and nOP. The chemicals were tested alone or on co-exposure with 25 pM E₂, which was set to 1. Mean values are shown (n ≥ 3).

*Significantly different from the respective solvent controls (cells + 0.1% DMSO; 25 pM E₂ + 0.1% DMSO).