Table 2.

AhR agonistic and antagonistic effects of the 4 test chemicals.

Concentration	BPA	BPA-DM	nNP	nOP
0.1% DMSO	1.03 ± 0.04	1.00 ± 0.05	0.98 ± 0.07	0.99 ± 0.04
60 pM TCDD	14.84 ± 6.26*	14.84 ± 6.26*	13.89 ± 7.32*	13.89 ± 7.32*
Compounds alone
1.0 × 10−8 M	1.15 ± 0.21	1.15 ± 0.23	1.30 ± 0.30	1.34 ± 0.27*
2.5 × 10−8 M	1.07 ± 0.21	1.11 ± 0.23	1.17 ± 0.22	1.27 ± 0.21*
5.0 × 10−8 M	1.11 ± 0.14	1.10 ± 0.08	1.30 ± 0.12*	1.18 ± 0.11
1.0 × 10−7 M	1.07 ± 0.20	1.12 ± 0.25	1.19 ± 0.13*	1.20 ± 0.20
1.0 × 10−6 M	1.22 ± 0.36	1.02 ± 0.09	1.47 ± 0.20*	1.08 ± 0.17
1.0 × 10−5 M	1.43 ± 0.33	1.37 ± 0.27*	3.91 ± 1.23*	1.00 ± 0.29
2.5 × 10−5 M	1.20 ± 0.37	1.15 ± 0.28	6.19 ± 2.22*	0.79 ± 0.25
5.0 × 10−5 M	0.73 ± 0.12*	0.86 ± 0.16	7.99 ± 1.64*	0.41 ± 0.03*
1.0 × 10−4 M	0.46 ± 0.15*	0.97 ± 0.25	3.68 ± 0.20*	0.54 ± 0.19*
Compounds + TCDD
60 pM TCDD	1.08 ± 0.19	1.10 ± 0.25	0.98 ± 0.03	1.04 ± 0.06
1.0 × 10−8 M	1.14 ± 0.15	1.44 ± 0.33	1.17 ± 0.24	1.18 ± 0.12
2.5 × 10−8 M	1.13 ± 0.06	1.48 ± 0.60	1.13 ± 0.16	1.22 ± 0.19
5.0 × 10−8 M	1.34 ± 0.33	1.53 ± 0.45	1.19 ± 0.13	1.12 ± 0.09
1.0 × 10−7 M	1.23 ± 0.11	1.64 ± 0.62	1.14 ± 0.19	1.18 ± 0.15
1.0 × 10−6 M	1.18 ± 0.27	1.57 ± 0.56	1.22 ± 0.22	1.15 ± 0.14
1.0 × 10−5 M	1.40 ± 0.37	1.86 ± 0.76	1.34 ± 0.35	1.24 ± 0.20
2.5 × 10−5 M	1.36 ± 0.42	1.44 ± 0.35	1.46 ± 0.38*	1.34 ± 0.45
5.0 × 10−5 M	0.93 ± 0.16	1.23 ± 0.21	1.41 ± 0.36*	1.10 ± 0.39
1.0 × 10−4 M	0.44 ± 0.13*	0.56 ± 0.29	0.61 ± 0.22*	0.41 ± 0.11*

Concentrations up to 10⁻⁴ M were not cytotoxic to the cells, whereas concentrations > 10⁻⁴ M elicited cytotoxicity to the Hepa1.1 2cR cell. The results given refer to effects observed at concentrations eliciting no cytotoxicity (n ≥ 3).

^*p < 0.05, versus the respective control, which was set to 1.