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Analysis of cell death in Physcomitrella. Evans blue staining of protonemal tissues after treatments with LB (A), CF(SCC3193) (B), CF(SCC1) (C), PDB (D) and B. cinerea spores (E). A closer view of CF(SCC3193)- (F), CF(SCC1)- (G) and B. cinerea inoculated (H) protonemal cells is shown. Arrows indicate cytoplasmic shrinkage. Leaves treated with CF(SCC1) (I) and B. cinerea spores (J) were also stained with Evans blue. Pictures of representative tissues were taken 2 days after treatment.
