. 2007 Aug 28;6:29. doi: 10.1186/1475-2859-6-29

Table 5.

Bacterial strains and plasmids used in this study.

Strain and Plasmids	Characteristics	Reference
Strains
E. coli DH5α
E. coli E10
L. lactis NZ9000	MG1363 pepn::nisRK	[35, 36]
L. plantarum WCFS1	Sequenced wild-type strain single colony isolate of NCIMB8826 form human saliva.	[10]
NZ7100	L. plantarum WCFS1 derivative with chromosomal integration of pEMnisRK plasmid.	This work
NZ7606	Cm^R, L. plantarum NZ7100 derivative carrying the pNZ8150 plasmid.	This work
NZ7601	Cm^R, L. plantarum NZ7100 derivative carrying the pMS011 plasmid.	This work
NZ7607	Cm^R, L. plantarum WCFS1 derivative carrying the pNZ7021 plasmid.	This work
NZ7602	Cm^R, L. plantarum WCFS1 derivative carrying the pMS040 plasmid.	This work
Plasmids
pUC18Ery	Amp^R, Em^R	[26]
pACYC184	Cm^R, Tet^R	[37]
pNZ84	Cm^R, pACYC184 derivative with deletion of Tetracycline resistance gene and BamhI site.	[38]
pNZ9521	Em^R, nisRK cloned in pIL253, expression of nisRK driven by rep read-through.	[27]
pNZ7130	Amp^R, Em^RpUC18EM derivative carrying 1.0 kb DNA fragments of both L. plantarum WCFS1 lp_0075 and lp_0077 genes.	This work
pNZ7131	Amp^R, Em^R, pNZ7130 derivative with extra cloning sites Nsa1 and NsiI.	This work
ppNZ7132	Cm^R, Em ^R, pNZ84 derivative carrying 1.0 kb DNA fragments of L. plantarum WCFS1 (lp_0075) and (lp_0077) genes and the Ery^Rresistance marker.	This work
pNZ7133	Cm^R, Em ^Rp7132 derivative carrying the whole genes nisRK from L. lactis.	This work
pNZ8150	Cm^R, pNZ8148 derivative lactococcal cloning and expression vector with nisA promoter upstream of a multiple cloning site and Sca1 restriction site.	[39]
pMS011	Cm^R, pNZ8150 derivative carrying L. plantarum (lp_0761) trxB1 gene, translation fused to the nisA promoter.	This work
pNZ7021	Cm^R, pNZ8148 derivative carrying nisA::pepN.	[40]
pMS040	Cm^R, pNZ7021 derivative carrying L. plantarum lp_0761 trxB1 gene, translational fused to the pP_EPNpromoter.	This work