Table 1.
WIP1 amplification is associated with gain of chromosome 17q or i17q in primary medulloblastoma specimens
| Specimen | Copy number (FISH) | Amplification* | CNA (CGH) | |
|---|---|---|---|---|
| CEP 17 | WIP1 | WIP1 | Chr 17 status | |
| D283 | 2–4 | 4–9 | Y | i17q |
| Daoy | 4 | 3 | N | +17 |
| MCF-7 | 4 | >50 | Y | ** |
| MB13 | 2 | 6–7 | Y | −17p12-p13, +17p11.2-q25 |
| MB1 | 2–4 | 5–8 | Y | i17q |
| MB3 | 2–4 | 3–7 | Y | i17q |
| MB14 | 2–4 | 2–7 | Y | +17 |
| MB15 | 2–4 | 2–6 | Y | +17 |
| MB5 | 2 | 6 | Y | i17q |
| MB2 | 3–4 | 6–11 | Y | i17q |
| MB6 | 2 | 4 | N | i17q |
| MB28 | 2 | 2 | N | N/C |
| MB27 | 2 | 2 | N | N/C |
| MB4 | 2–3 | 2–3 | N | i17q |
FISH analysis for WIP1 and chromosome 17 centromere reveals low-level amplification of WIP1 in 7 of 11 primary medulloblastoma samples. WIP1 copy gain is significantly associated with gain of 17q (P = 0.00057)
*Amplification is defined as >4 copies as determined by FISH
**Reportedly amplified, but not determined in the present study
Abbreviations: MB, medulloblastoma; +17, gain of chromosome 17; −17, loss of chromosome 17; N/C, no change in chromosome 17; CNA, copy number aberration; CEP 17, centromeric chromosome 17 probe